FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_323_rr.352000000a9248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_323_rr.352000000a9248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8240621
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA110250.075.8769451
TAGTAAC109100.075.828772
CGCACTT29800.068.978995
GTAACAA146550.056.494394
AGTAACA162350.051.2610933
ACGCACT44300.048.4827464
TAACAAG172150.048.0094385
GCACGCA45400.045.5947532
AAGAGCG37000.043.4357269
AGCACGC47600.043.340351
AAACACG72100.041.7819565
ACAAGAG226850.036.423267
AACACGA76850.034.8257066
CAAGAGC229950.034.4836168
CACGCAC62750.033.551723
AACAAGA260650.031.6732626
GTAGAAA170200.031.1981371
ATTAGTA71900.030.4674431
ACACGAG89450.029.7614577
ACGAGCC32550.029.5808669
Produced by FastQC (version 0.11.2)