FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_310_rr.352000000a923b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_310_rr.352000000a923b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6595791
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA82800.061.3087271
CGCACTT30850.059.2831845
TAGTAAC91350.053.939792
ACGCACT40400.047.376464
GTAACAA116550.042.515284
AAGAGCG42450.041.534329
AGCACGC45400.041.3837281
TAACAAG121100.040.741045
AACACGA41950.040.3426676
ACGATCC8600.038.354523145
AGTAACA130400.038.3293383
AAACACG51550.037.5405775
GCACGCA52150.036.1626552
ACACGAG50550.033.4792257
ACGAGCG6200.031.9780399
ATTAGTA69750.031.917221
CACGAGC54000.031.0770688
ACAAGAG169550.029.2347167
CACGCAC69950.028.3785463
GTAGAAA126350.028.000441
Produced by FastQC (version 0.11.2)