FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_297_rr.352000000a922e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_297_rr.352000000a922e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6915188
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG190460.27542273615699236No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT186950.2703469522448269No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT175910.25438209344416957No Hit
CTGTTGCTGGGCCCATCCCATTGTTAATCATATTGTTCTTTATTATTGTC158270.22887302557790187No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC154760.22379724166573636No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA130760.18909102688169865No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT123010.17788381169101983No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC117820.17037859274397169No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG111760.16161527351100216No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA102540.148282302664801No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT101890.14734234268106666No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT99870.1444212362700768No Hit
CCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT92950.13441427767401262No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA89770.12981570421512761No Hit
ATTGCTACTTCTTTCATTATTTTCTGGTATGTTAATCCATCCCCTGTGGC87490.12651861381064405No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT80200.1159766010699926No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG74920.10834123381750431No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT72430.10474046403366041No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT72340.10461031572822024No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT72290.10453801111408685No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGG1250.098.136024145
GAAACAC45850.094.470544
AAACACG46250.093.496835
CGGTGCA11500.093.091122
AGCCACC8750.085.19502145
AGCACGC5300.081.599861
GTAACAA123600.076.110924
TCGCGCC652.2914428E-672.58582145
AACACGA55200.072.47186
GCACGCA6100.070.897212
AACACGT4600.070.8043066
CGGCGTT400.002130718770.77118145
GGTGCAT15250.070.1963043
GCGGAGC21450.066.766641
CACGCAC6650.065.0302353
ACACGAG62900.063.486117
TAGTAAC150600.063.0825162
CGCACCC902.1469168E-762.907715145
ACAAGAG150300.062.7473687
ACGCACT6900.062.672254
Produced by FastQC (version 0.11.2)