FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_291_rr.352000000a9211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_291_rr.352000000a9211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8276135
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA84000.054.692221
TAGTAAC86400.051.120012
ACACGAG33600.042.9877747
AAACACG41850.039.805135
CGCACTT20050.038.8906335
TGACGCC9400.038.17438145
AGTAACA121800.036.9016233
ACGCACT22700.036.849874
GACGAAA15150.036.775253145
AACACGA40050.036.5956736
GTAACAA124950.035.6289674
TAACAAG129600.034.349575
CACGAGC43650.033.0880438
TTGCGCG1751.2453529E-732.3641329
CTGCGCG10000.032.106667145
AGCACGC28050.030.5831551
AAGCGGT16300.029.1032357
GCACGCA30200.028.1702652
CAAGAGC156950.027.1556598
CGAATAA20350.025.521994145
Produced by FastQC (version 0.11.2)