FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n02_s2_137_rr.352000000a9204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n02_s2_137_rr.352000000a9204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7648645
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT194470.2542541848915723No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC165710.2166527535269319No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA152700.1996432047767938No Hit
ATCCTTTTGTTATTCCAACTTCTATAAATCTCTTGGTTTTATAATCAAAC106630.1394103138529766No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT103330.13509582416231897No Hit
GTACTAAAGGAAGAGATGGTCTGTTAGTGGCAGATGGTGGACCCAACATT98320.12854564435922966No Hit
GTTAGCTATTATCGTTTTGTGAAACAATATGTTATCATCAATTCTTTTAC97840.1279180822224067No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA94880.12404811571199865No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC94800.12394352202252817No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT91020.11900147019504762No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA87700.11466083208202238No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC86600.11322266885180317No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT82920.1084113591361607No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC81860.10702549275067674No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA80090.1047113573711422No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAACCTCATTTAGGTTGAA78400.10250181568107815No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG78100.10210958934556383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG34700.067.208655
TACGCGG1351.4551915E-1162.95204145
CGCACTT10850.059.452545
AGCACGC10900.059.1891331
ACACGAG34550.058.6075177
AACACGA34900.058.4262666
GAAACAC41900.056.5074124
ACGCACT11750.054.900534
CACGAGC36900.054.4888278
GCACGCA12150.054.2653162
CGGTGCA12750.053.9358182
ACGAGCC17450.053.1481939
AGCCACC34400.051.05704145
ACGATCC3200.047.21403145
TAACAAG131300.047.0771875
TGACGCC4600.045.16125145
TAGTAAC138950.044.746262
GTAACAA138850.044.7230264
AGAAGCG19150.044.049095
CGCACCC2058.62201E-1041.456226145
Produced by FastQC (version 0.11.2)