FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_pr8_cw_rr.351000000a93b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_pr8_cw_rr.351000000a93b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647585
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG468450.7046920046904253No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA322860.4856801379749187No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG223970.33691934740210167No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA164570.2475635888822783No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT152440.2293163607535669No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC137480.2068119474967225No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG130310.19602607563498622No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT119340.17952384211709968No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT116880.17582325009759184No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA113340.17049800792317812No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT104710.15751584974092095No Hit
TCCTCAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG104100.15659822326453893No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT103410.15556025233223794No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT102000.15343918129666637No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC100950.1518596603127301No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT99220.14925721145348272No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT97470.14662467648025562No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA97200.14621851394152913No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG92770.13955443969501707No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA92070.1385014257057262No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA91010.13690686166480007No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA90940.13680156026587098No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC87340.13138605974951806No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC85740.12897917063113898No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT85440.1285278789214429No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT85220.12819693166766577No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA81610.12276638809432297No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA80200.12064531705875141No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA79920.12022411146303508No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT77590.11671907918439554No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT75070.11292822882294849No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA73630.1107620286164073No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA72980.10978422991206581No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG71660.10779854638940306No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT71610.10772333110445373No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT69440.1044589877376521No Hit
TCCTCAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGGCCCA68720.1033758876343815No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG68070.10239808893004002No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA67740.10190166804937431No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA44100.0141.390531
GGCTCGG203.8669267E-4108.750723
GCAAAAG225950.0104.892263
AGCAGGC17500.0102.741988
ATTAGTA6700.093.0645141
GCAGGGG51200.090.624249
ATTATCG406.121609E-790.624245
GCAGGGT146250.089.130879
AGCAAAA269400.088.274132
AGCGAAA70700.087.7832262
AGCGTCA502.2704626E-887.000573
AGCAGGG216150.081.874168
GGCAGCC801.8189894E-1281.56181145
AAGCAGG375700.079.677937
TATTAGT2800.077.682591
ACTAATA3950.077.087958
TACTAAT3950.077.087957
GAGCAAA312000.076.9190441
CAAAAGC315800.075.507944
ACAGGTC903.6379788E-1272.503751
Produced by FastQC (version 0.11.2)