FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_pr8-vww_100-1_rr.351000000a934a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_pr8-vww_100-1_rr.351000000a934a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5145560
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG340190.6611330933853653No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA203730.3959335815732399No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG182190.3540722486959631No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA106280.2065470036303143No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC105420.20487565979213146No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG102410.1990259563584916No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT101470.1971991386748964No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA97920.19029998678472315No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG94840.18431424373634744No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA94180.18303158451169552No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG91290.17741509184617418No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT90420.17572431377731482No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT86770.1686308195803761No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT86350.16781458189196122No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT83030.16136241730734846No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC81900.15916634924089895No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA80660.15675650463700744No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT72000.13992646087111996No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT67550.1312782282200577No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT66910.13003443745675886No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC66210.12867404130940074No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC65030.12638080208956848No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA64630.12560343286250672No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG64410.12517587978762273No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA62670.121794323649904No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA61800.12010354558104464No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA59110.11487573752905417No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT58390.11347647292034298No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT58140.11299061715342937No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA57320.1113970102379527No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT56920.11061964101089093No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT56590.10997831139856497No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT56220.10925924486353283No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT55640.10813205948429325No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA55120.10712147948911295No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT53720.10440068719439673No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG52540.10210744797456447No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA52340.1017187633610336No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG52110.10127177605547308No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA52080.10121347336344343No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA27600.0141.857151
GCAAAAG154200.0107.106243
GCAGGGG39800.093.0821469
TCTACTA4700.092.551185
GCAGGGT114700.089.690729
ATTAGTA6950.088.674891
AGCAGGC7800.088.2993558
AGCAAAA187900.087.937812
AGCAGGG168400.083.347358
AGCGAAA47150.082.729172
TACTAAT5450.079.814777
CGCTCCC1100.079.089196
CTACTAA5650.076.989476
AAGCAGG267600.076.453867
ACTAATA5750.075.650538
GAGCAAA225350.074.451311
CAGGTCG300.001935695672.504761
CAAAAGC229850.072.200884
AATACTA6550.071.949892
TTAGTAG9200.066.986792
Produced by FastQC (version 0.11.2)