FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_pr8-vww_1-5_rr.351000000a938d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_pr8-vww_1-5_rr.351000000a938d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6601378
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA223910.3391867576739281No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG112880.17099460143018624No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT88840.13457796235876812No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG80920.12258046729031423No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC66990.10147881245400582No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA66030.10002457062752655No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCCG100.0070920964144.997675
CCGTACG100.007092257144.996578
GAGCGAA7650.0139.322041
AGCGTCA458.54925E-11112.780233
GAGCAGA8000.0110.569091
CACGCAC900.0104.7244953
GCACGCA1050.089.766592
GTAACAA76600.087.929474
AGCACGC1100.085.686931
CGGAGCA9650.083.3981252
GCGTCAG801.8189894E-1281.567371
GCGGAGC13850.076.954051
ACGTGCA3150.073.654632
GGTTACC300.00193640672.49828145
AGGATTG48950.067.9815445
AAACACG42450.067.802215
TAGTAGA8100.066.236013
CGGTGCA12150.065.044542
AACACGT2850.063.595476
AGGGGTC350.003567616262.1418535
Produced by FastQC (version 0.11.2)