FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_pr8-vww_1-1_rr.351000000a9370.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_pr8-vww_1-1_rr.351000000a9370.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6094072
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG231870.3804845101928563No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA137610.22580960645033404No Hit
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA129640.21273132316126228No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG121530.19942330842169245No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA74520.12228276922228683No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC73480.1205761927328722No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG72020.1181804218919632No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA69650.1142913966228164No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA68560.1125027731867953No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT68130.11179716944597963No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG66390.10894193570407439No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT61640.10114747577645948No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT61410.10077005982206971No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGC100.007090549145.008161
GAGCGAA18100.0138.999531
GAGCAGA4400.0115.3474041
CGCTCCC750.0106.334956
AGCACGC451.0921212E-896.672111
GCGTCAG950.091.584111
GCAAAAG123950.086.56923
GCAGGGT83050.082.0582359
CCTCGCC451.2336513E-680.555466145
AGCAGGG118500.077.210258
ACGTGCA2850.076.319462
CACGATG300.001936036772.50173
GTAACAA49350.071.1060264
TCTACTA4250.069.9422455
GCGGAGC6750.066.5963441
CAAAAGC164000.066.0030754
CGGAGCG1150.063.0465052
AGAAGCG6250.062.6409535
GCACGCA702.3357461E-762.1458442
AAGCAGG221100.060.0399977
Produced by FastQC (version 0.11.2)