FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_pr8-vww_1-10_rr.351000000a939a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_pr8-vww_1-10_rr.351000000a939a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262659
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA212580.4039402895000417No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG91760.17436052763441448No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT86280.16394754058737226No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC63180.12005337985987691No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT60940.1157969763953925No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA59860.11374478186787326No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT58280.1107424972813173No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT57920.11005843243881087No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT57320.10891832436796683No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT55400.10526997854126592No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA54480.10352181283263841No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC54210.10300876420075858No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT53430.10152662370866135No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCGG100.007091061145.004476
TGCGTAC100.007091061145.004473
GAGCGAA4050.0134.269781
GAGCAGA7550.0122.9235461
GTAACAA69000.093.097074
AGGATTG36050.089.697635
CGGTGCA10000.083.3799442
AGCGTCA353.4381166E-582.8596953
AACGGCC353.438603E-582.85733145
TAGTAGA7000.074.573723
ACAGGGT502.306535E-672.505681
CACAGCG406.664806E-572.5022353
GAAACAC39100.071.760524
TCTACTA2600.069.7136845
CGGAGCA9250.068.9770662
GGATTGA47250.067.975636
TAACAAG96350.066.8209465
AAACACG41950.066.366775
GCGTCAG554.06212E-665.914251
AGCACGC554.06212E-665.914251
Produced by FastQC (version 0.11.2)