FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_pr8-vww_1-100_rr.351000000a93a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_pr8-vww_1-100_rr.351000000a93a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5852777
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA257380.43975705891408473No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT101640.17366115264600035No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG96340.16460562225418804No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC80270.1371485706699572No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA74360.1270507999877665No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT73360.1253422093478019No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT72650.12412910999342705No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT71840.12274515157505574No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT70160.11987471929991525No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA69420.11861036222634144No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT68830.11760229374876234No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT68010.11620124942399138No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC67520.11536404001040873No Hit
GTACTGGCCAGCCATCTTCTGGGGGATTATGCTTTCAAATGCTTCAAATG63550.10858093516974933No Hit
GTATTACCCAAGGGTTGTTACTAATACATTCTTCTATTCCAGAATACATT61760.10552255792421272No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTTATTGAATTAATGGAT61480.10504415254502264No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG59200.10114856588590339No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC58560.10005506787632606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATTG32650.0118.350765
GAGCAGA8600.0113.816931
CGGTGCA9250.0105.032452
GAAACAC35150.099.829344
AGCGTCA451.0926669E-896.66673
AACACGT2650.095.75236
GTAACAA83050.090.526824
AGCACGC1050.089.768841
GCGTCAG1150.088.2676851
GGACGAC259.389984E-486.99786
GCACGCA1100.085.686242
CACGCAC1150.081.956563
TAGAAAC48800.079.783832
GGATTGA48700.078.452846
AACACGA43950.074.065256
AAACACG48800.072.201045
TAGTAGA8500.069.94123
ACAAGAG111800.067.4401557
ACACGAG48500.067.116867
ACGTGCA4350.066.6701052
Produced by FastQC (version 0.11.2)