FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_lp_ctrl_rr.351000000a93dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_lp_ctrl_rr.351000000a93dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3068
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT652.11864406779661No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG140.45632333767926986No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC100.3259452411994785No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT60.1955671447196871No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG40.1303780964797914No Hit
GGATCTTAGGAGAGGCTTGCTGGGAGATGGAGAATATGGAGGAAGGGGGG40.1303780964797914No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT40.1303780964797914No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG40.1303780964797914No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG100.006750369145.06
GCAGAAA100.006750369145.01
AAAAAAA1706.9973455E-48.52941165-69
Produced by FastQC (version 0.11.2)