FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_extraction_neg_ctrl_rr.351000000a933d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_extraction_neg_ctrl_rr.351000000a933d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451260
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGCGCAGGCGTCGCTGCACCTGCCGCTGTGTTCGC12230.27101892478837036No Hit
GTAGAAACACGAGCACGAACACGAGCACGAACACGAATGCGAATGCGAAT10060.22293134778176663No Hit
TTACAGAGCAAGTCAAAACCAACTGGGACCCAACAAGAAAAAAACAGAAT9430.2089704383282365No Hit
CTGTAGGGTGGAGTCTGAATTCCAAGAATTGAGTTCCCCATCTGTTAAGC7420.16442848911935468No Hit
ATCTGCAACAGATCAAAGAAACAAAAGTGAAAACATGGAAGCCAGGGATG6630.14692195186810264No Hit
ATATATGTGTATGTAACTGGATGATGTCCTCTGGTCACAGTTTTACTTTC6260.1387226875858707No Hit
CTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAGAGACACTA6230.13805788237379782No Hit
ACCGTAAACCTTGCAAACATAAGGATTATCAAGACAATTTCTTCTGACTC5960.13207463546514203No Hit
ATAATATTCCTACCTGTGACTAATTTGGTGTCTGTAGGGTGGAGTCTGAA5230.11589770863803571No Hit
GTTGCAGATGGTAGTGTTTGTTAACTCTGTCTGGTTTCCTGGTTTCATTG5090.11279528431502903No Hit
CAATAGGACAGTGAATTGCAGTTATATCAGCATTACTTGACCAGTTCTAT5010.11102247041616807No Hit
GTCTTATGCAGGAATTTGGAGGTGACAGTAATAGCAGGATTACCGTAAAC5010.11102247041616807No Hit
GTATTAACTAGTTTACAGGAAATGAAGCACTTCGTACTGTGCATTTGCAT4970.11013606346673757No Hit
GCATAAGACAATCAATTTTGGGGGGAAAAAATCCCCAGTGTTCCCATAGT4960.10991446172937996No Hit
AGTTAATACATTGGCTGTGGTGATGACTATGGGAACACTGGGGATTTTTT4890.10836324956787662No Hit
GTCAAAGAACAGATATCGAACTACATAGAAAGTGAAACTGTGGGCCGGGC4710.10437441829543943No Hit
CTCTAGTGGAGAATTTTAATTCAGGGCTAGTGCAGAATCATTTTTTTCTG4560.10105039223507511No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC100.0070902617144.99535145
TAGGACG301.5987587E-596.663564
GGTGCTA1050.075.949953
CGCACTT1550.074.83635
ACGCACT1650.074.694574
AGCACGC1650.074.694571
ACGTGCA1300.072.497689
GCACGCA1700.072.497672
GACGAAG406.6602865E-572.497677
AACACGT1850.066.6194846
ACACGTG2050.060.1200187
AAGAGCG1400.056.9624569
CACGCAC2350.055.5301323
AGCACAG2000.054.3732571
AGTAACA13950.050.9302643
ACAGCTA2200.049.430234
ACGTGCG604.942961E-448.331789
TAACAAG14950.047.5235635
CACGTGC2600.047.402338
AAACACG15950.047.271215
Produced by FastQC (version 0.11.2)