FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_428_rr.351000000a9313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_428_rr.351000000a9313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5535549
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC58400.10549992421709212No Hit
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT55810.10082107483828613No Hit
ACAACAGCTTGTGCAAAGGCCCTGAGGCATGAATGATTAAAAGATATAAT55630.10049590383898689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT33250.091.1420445
TAGTAAC108850.089.654952
GTAGTAA113900.086.062621
GTAACAA125300.077.997524
TAACAAG156950.062.4062585
CTCGCTC34750.062.1732333
TCTCGCT36450.060.8669552
GCACGCA51350.060.0075842
TCGCTCT37550.057.344074
AAGAGCG22350.056.442459
CGCTCTG37950.056.3565445
AGTAACA178600.054.801733
ACGAGCG4850.053.8139539
TCTGTCG43350.051.3432588
GTCTCGC47050.047.9250261
ACGCACT66500.047.5342674
ACAAGAG207150.047.1079567
AACACGA63400.044.9432951
AGCACGC69750.044.2819751
AGAGGAT95900.043.696963
Produced by FastQC (version 0.11.2)