FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_425_rr.351000000a9306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_425_rr.351000000a9306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6372173
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT163780.25702378136940096No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG156300.24528524256952847No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT124900.19600848878396743No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA115290.18092729120819537No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA111750.1753718864820525No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC93490.14671604176471667No Hit
CCCCTTAGGTGGACCCTAAACTCTTCACTATAACTTCCCTGCCCAGCACC92760.14557043570537084No Hit
CCTAAGGGGTAGCCACTACTCAGCACCCACTTTTTGTTGCCAGACCTTCC91870.14417373790699028No Hit
CCCATATTCCAGGCCCTCCCACAGCCCTCACCCGGTCAGTCACCCCATCT89950.14116063703857382No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT87200.1368449977739148No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG85130.13359649840015328No Hit
ATATTGTAGTTGGGTCTCTACAGTGAGGCAGGATCTGCCCAGGCAGAGGG83930.13171331035739298No Hit
GTAGTAACAAGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC83910.13168192389001365No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA81390.12772722900021705No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG76980.12080651294307296No Hit
CATTTAGGTTTCTGGTCTCTGATAATTTGGAAGGTCTGGCAACAAAAAGT76930.1207280467746246No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG76780.12049264826927958No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA75790.11893901813400233No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGAAATAGTATGTTATCATC74790.11736969476503542No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC73350.11510986911372305No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA72650.11401134275544622No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT72630.11397995628806688No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG71800.11267741789182434No Hit
GTCCAGAAGAGGGTGAAAGGAACCTCCACTTCGGCCATTGTGGAGGCTTA71240.11179859680520286No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA70830.11115517422392643No Hit
TCACAGAGCACCCCCTAGTCTTGGAAACCCTTCCTCAAAGAGTCACCTGG70330.11037051253944298No Hit
GTCTTGGGCAGGGCCTGAGAATGTGCGTTTCTAACCAGTTCCCAGGTGAT70220.11019788696885661No Hit
GGCTTAGATAAGTAGTTCCAATACCAGGTGCAATAGGGGAAGGAAAAAGT69780.10950738468651117No Hit
GCCCTCACCCGGTCAGTCACCCCATCTGTGCAGGGCAGTTTGCTGCCCCC69280.10872272300202772No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT67450.10585086123681828No Hit
CAGTAAAGTCTGAGCTAAAATCGGAGGCTCTCGAGCCACATCCACCTCGC66490.10434431080261004No Hit
AATTAAAACAAGGGGTGATGGGAAGAATCACAGGTTGTGGCCCCGGGGTT66230.10393628672667865No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC66130.10377935438978195No Hit
CTACAGTGAGGCAGGATCTGCCCAGGCAGAGGGAAGAGCAGGCAGAGCCG64790.10167646107536628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT18000.082.9729165
GCACGCA18650.082.418742
GTAACAA90800.078.2507864
TCGACTG300.001936156572.500615
CGGTGCA9900.065.913792
AAACACG38900.065.604665
TAACAAG109000.065.250555
AGCACGC23900.064.011831
TAGTAAC116650.063.088512
ATTAGTA39450.060.4678651
GAGCATC19900.060.1145174
GAAACAC45250.056.8798264
ACACGAG40200.055.7276427
ACGTGCG3400.055.4451262
ACAAGAG129750.054.8709377
AGTAACA132150.054.808363
GCGAACT400.00605235754.3750347
CGGACGG400.00605254554.374607145
CGTGCGT3000.053.1679463
ACGAGCC7550.052.8137789
Produced by FastQC (version 0.11.2)