FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_412_rr.351000000a92f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_412_rr.351000000a92f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5440985
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG229570.4219272797113023No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG111690.20527533158058697No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT85510.15715904381283904No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT82060.1508182801459662No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG79520.14615000776513812No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG79090.14535970968491918No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA77190.1418676949118588No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA73280.13468149608940294No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT70850.13021539298490992No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG70830.13017863493466716No Hit
GCACAGCATTTTCTTGTGAACTTCAAGTACCAGTAAAAGAACTGAAAATC57700.10604697495030771No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGT57500.1056793944478803No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC57390.10547722517154523No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC56700.10420907243817067No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT56010.1029409197047961No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA54440.10005541276074092No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGG850.0110.8826145
CGGTGCA21650.0109.173842
AGCACGC9600.097.426561
GGTGCAT24800.095.594963
CGCACTT10150.091.426255
CGTCAGA900.088.615372
ACGCACT10600.088.231324
GCACGCA12350.076.3194662
GCGTCAG1000.072.503491
GCGGTGC37850.070.3006061
GCGGAGC8050.069.351161
GAAACAC33250.069.120454
GTAACAA100800.066.890034
ACGTGCA4450.063.542382
ACGAGCG350.00356771462.1412739
AAACACG37550.061.5896455
TAACAAG114400.058.999815
GCAGAGC70300.058.16781
AACACGA36950.057.6845976
ACCGACC1650.057.1213343
Produced by FastQC (version 0.11.2)