FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_395_rr.351000000a92de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_395_rr.351000000a92de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4477045
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT95930.21427079692073678No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT88920.19861314773472233No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA81580.18221840522040764No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC75410.16843699359733932No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT59070.13193970576574504No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT56510.12622164843105219No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT54570.12188843310710525No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC53980.12057059958075025No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA53650.11983350625245No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC52650.11759989010608561No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC52590.11746587313730374No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT51070.11407077659482984No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC50780.11342302791238418No Hit
GGAACAGGCTACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAA49820.11127875641187435No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA46530.10393015929033547No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT46400.1036397891913081No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA56700.0115.337354
CGGTGCA12650.0109.473432
ACGAGCG203.8666453E-4108.752114
AGCACGC3400.0102.360651
GAAACAC28550.097.007144
AAACACG29050.095.584925
GGTGCAT14550.093.6819463
TAGAAAC33200.089.10212
TTCGGAA1000.079.748886145
ACGCACT4700.078.6717454
CGCACTT4700.078.669995
TAGTAAC85300.078.0296252
TAACAAG85600.076.480515
GCACGCA4650.074.843512
CGGAGCA10700.074.537492
AGCGTCA608.035204E-872.5038453
AACACGA35450.072.397546
GCGGAGC14950.071.292991
ACAAGAG93650.069.9063647
GAGCAGA11300.066.730681
Produced by FastQC (version 0.11.2)