FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_394_rr.351000000a92c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_394_rr.351000000a92c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7531394
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT214060.2842236111933594No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG201640.26773264019914506No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT195770.25993859835244315No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA167670.22262810842189373No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC155270.20616369293652675No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA152010.2018351449944061No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC151570.20125092379976403No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT148430.19708170891072754No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC117090.15546922654690484No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG116950.1552833379849733No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG114330.15180456632596834No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG113590.1508220124986158No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA113410.1505830129189895No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA112890.14989256968895798No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT107160.1422844164041876No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA103940.13800897947976165No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG99880.13261821118374634No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT95990.1274531647129336No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG95950.1274000536952389No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT94310.12522250196975487No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT93820.1245718920029944No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA93280.12385489326411551No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC93010.12349639389467608No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT91950.12208895192576566No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT89690.1190881794260133No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC89300.11857034700348966No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG88970.11813218110750812No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC83480.11084269392890612No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG83060.11028502824311144No Hit
GATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG82840.1099929176457904No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT81990.10886430851977735No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA81590.10833319834283002No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGTCAAGAATAAA79090.10501375973690925No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG78980.10486770443824875No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT78660.10444281629669089No Hit
AGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCG76910.10211920927254636No Hit
AAATACAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGA76340.10136237727039642No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAA76120.10107026667307539No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA75450.066.303224
GAAACAC23750.064.411544
AAACACG23850.063.5326425
AACACGT3900.061.34636
TAGTAAC93400.055.7368662
CACGAGC22850.055.5245558
TAACAAG91600.054.5334245
ACGAGCC8200.050.395799
GAGCAGA28450.050.2062261
GCGAAGC450.0096384448.3372961
CGAAGCG450.00963919948.3363342
ACGAGCG2700.048.3328069
GGAGCGT1255.2041287E-946.400116
AACACGA28800.045.564346
ACGAGCA15550.044.7583549
AAGCGGA5800.041.2525632
CGTCAGA1252.7207716E-740.6025242
CGGAGCG1451.9306754E-840.0000955
ACACGAG32850.039.946827
ACGTGCC2005.456968E-1239.8745659
Produced by FastQC (version 0.11.2)