FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_379_rr.351000000a92b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_379_rr.351000000a92b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4007680
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT85480.21329048227403385No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC72190.18012915202810603No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT61940.15455325774512935No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT60540.1510599648674545No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT56920.1420273075694666No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT54140.1350906259980837No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC53900.13449177579048227No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA50370.12568368732034493No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT49920.1245608431810923No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC49220.12281419674225486No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT46830.11685064675822422No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT45610.11380649153625039No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC44300.11053776748642606No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT44300.11053776748642606No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT42060.10494849888214627No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC41440.10340146917917599No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA6050.0125.835191
ACAGGCG351.902663E-9124.2943341
AGCACGC1650.0114.250351
CGGTGCA9250.0112.8726962
GTAACAA56450.0112.767114
GGTGCAT9350.0111.661333
GAAACAC23450.0107.284894
AAACACG25150.0100.0305255
TTCGGAA800.099.6882145
TAGAAAC27700.096.062632
ACAGGTT406.1183164E-790.6312941
ACGTGCA2600.089.236972
GCACGCA2150.087.6805042
CACGCAC2350.080.215333
TAACAAG80500.079.435345
CGGAGCA9950.078.698932
AACACGA30950.078.0053946
CAGCATC4500.077.335814
TAGTAAC85200.076.164332
GCGGAGC14750.072.750821
Produced by FastQC (version 0.11.2)