FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_363_rr.351000000a92a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_363_rr.351000000a92a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6554125
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATGCTGCCTACAAGAAACTCACTTCACCTACAAAGACATACATAG89640.13676882879102856No Hit
AGCTAGAATAGTACCTTATGTTGCTGGCTCTGTTTCATGTAAGACTGATA66300.10115766788091468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG39950.065.149755
ACGAGCC17700.058.1630069
ACGAGCT11700.057.0077259
CACGAGC41250.053.0780268
AACACGA42400.051.8097886
ACACGAG42600.051.5665557
ACGCACT11650.050.408544
CGCACTT9650.050.336445
CAGCACG13000.044.05844
ACGAGCG2350.043.190879
GCACGCA16000.035.799092
CACGCAC17500.033.5576823
GAAACAC82550.033.1107674
ACACGTG13700.032.2808047
CTGCCTA54800.030.9575829
GCACGCT18750.030.5464576
GCGTCAG1204.058104E-430.2113551
TAGAAAC137600.029.7184092
ACGAACG1504.4025935E-528.9998055
AGCGACG1000.006077086628.9998045
Produced by FastQC (version 0.11.2)