FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_361_rr.351000000a929b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_361_rr.351000000a929b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5228559
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG141960.2715088421111821No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT123720.23662351328540043No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC111330.21292673564551914No Hit
GTATGCACTCTGTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTC97710.18687749339732038No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT77770.14874079072264462No Hit
GTGCTTGTGATTCTAATTCACTTACATCATTGGCTCCCATTATGACCTCC73790.14112875077052778No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT72520.13869978324811866No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC68810.1316041379661203No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG67190.12850576994540944No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT64060.12251941691773967No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG61460.11754672750178397No Hit
CCAATAGGGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT61280.11720246438837163No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT61230.1171068357457571No Hit
GCTTTATGGTCTGGCAGTTAAAGGACAATCTCATCTGAGAGGAGATACTG55850.10681719380043334No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT55010.10521063260450919No Hit
GCAGAAGCACAGCTAATGATAACATATGATACACCAAAGATGTGGGAGAT54810.10482811803405107No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC54390.10402483743608898No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA53560.10243740196868774No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT52960.10128985825731335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG18100.075.302885
CGGTGCA6350.067.365832
GCACGCA2800.064.735632
AACACGA20550.059.622096
CGCACTT2700.059.0732965
CACGCAC3050.057.0500683
CCGTACC400.0060517154.376366145
GAAACAC25700.053.600064
AGCACGC3450.052.5390621
CGGAGCC4200.051.7850388
ACACGAG23600.051.6094937
CACGAGC24150.049.8337178
ACGAGCC7750.047.7090539
AGCGGAG6600.042.8403476
AAGCGGT12250.042.6116877
CAGGATT11350.042.1580355
AGAAGCG15150.042.111665
CAGAAGC41150.042.1104052
ACGCACT4350.040.000624
AACACGT3650.039.7255066
Produced by FastQC (version 0.11.2)