FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_338_rr.351000000a928e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_338_rr.351000000a928e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5522606
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG67730.122641376190878No Hit
CGATTGGAGTTATCATTGTTCCTTTCAATTTTTCTCTTTTTTCTTTTATC64510.11681079548314692No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG62250.11271852455163377No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG59710.10811924660205707No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA57420.10397265349003713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGCG703.092282E-1182.860823
AAACACG17900.075.3371665
AACACGA16550.072.282866
GAGCAGA18350.071.124181
AACACGT2350.067.8741156
GAAACAC20000.067.789894
ACACGAG17700.067.586527
CACGAGC17900.065.613878
CACGTGC2500.063.799368
GCAGCGT502.0100642E-458.0057221
ACACGTG2750.058.001527
ACGAGCC6550.056.4498149
CGGTGCA5550.056.17622
ATACCGA400.00605170254.3764236
ACGAGCA15500.053.789789
CGATTGG21650.053.2500531
ACGTGCA3050.052.2945569
AGAAGCG13700.048.6874055
CGCACTT1703.8198777E-1142.648185
AGCACGC2100.041.4326551
Produced by FastQC (version 0.11.2)