FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_337_rr.351000000a9271.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_337_rr.351000000a9271.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7808899
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA148150.098.8138051
TAGTAAC150900.096.531292
CGCACTT51350.096.289665
GTAACAA167550.087.278714
TAACAAG197900.073.8550955
AGTAACA198700.073.7055743
GCACGCA62200.073.672282
AGCACGC62000.073.443091
ACGCACT68200.073.351944
AAGAGCG43200.057.2273379
CACGCAC84800.055.060083
CAAGAGC290400.049.156628
ACAAGAG298750.049.0446177
AACAAGA310800.047.003456
ACGAGCG10550.043.9819374
AAACACG85350.043.831055
CTCGCTC34550.043.018243
AACACGA99600.042.4416431
TCGCTCT36000.041.285564
TCTCGCT38700.039.15732
Produced by FastQC (version 0.11.2)