FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_328_rr.351000000a9264.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_328_rr.351000000a9264.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6774962
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG149220.2202521578718818No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC119490.17636999292394553No Hit
GCTTTGGTCATGTCATATCCTTGTATCGATGATTCTTGTTCAACAATTGC87500.12915201590798592No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT83360.12304128052673949No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT81830.12078296527714841No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT79060.11669438145926131No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT78980.11657629961614543No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA76400.11276816017565855No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG74150.10944710833802464No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATCCTTTTAC73160.10798584552946569No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT69990.10330685249599925No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT69090.10197843176094566No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC68810.10156514531004011No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT68280.10078285309939745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG23750.077.536035
AACACGA24350.070.861696
ACGAGCG451.1945031E-464.442829
ACACGAG26850.064.263297
CACGAGC28800.058.9047668
ACGAGCC10100.058.8599059
GAAACAC33000.056.022974
AGCACGC4700.055.5350271
CGGTGCA10250.052.3443952
CGCACTT4350.051.6661265
AGAAGCG17450.049.0252765
AAGCGGT14550.045.841117
GCACGCA5800.045.002522
GAAGCGG20800.041.129386
CAGTGCG1451.9308573E-839.999585
CACGCAC6700.038.9553953
GTAACAA112200.037.0255624
TAGAGCG800.002037400836.2501564
ACGCACT7200.036.2501564
TAACAAG116000.035.6871265
Produced by FastQC (version 0.11.2)