FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_325_rr.351000000a9258.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_325_rr.351000000a9258.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7222447
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT27900.070.160625
AGCACGC38200.057.319381
ACGCACT39950.055.1695864
CACGCAC42750.052.0652773
TAGTAAC68800.051.847592
GTAGTAA80500.045.9337841
GCACGCA48800.044.719672
CACGAGC34800.041.4576578
ACGAGCG3550.040.84449
AACACGA38750.037.2319076
TAACAAG97900.036.731015
ACACGAG39600.036.4327357
GTAACAA104100.034.6833884
AAACACG53500.031.4389555
AGTAACA118000.030.843783
ATTAGTA70100.028.0291041
TAGACCG4150.027.951359
ACGAGCT15300.024.6401189
AGAAGCG31300.024.0892265
GTAGAAA133750.023.6347311
Produced by FastQC (version 0.11.2)