FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_323_rr.351000000a924b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_323_rr.351000000a924b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8240621
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC122600.084.98182
GTAGTAA124750.084.97171
CGCACTT37450.083.0518345
GTAACAA162150.063.9836654
ACGCACT53800.060.7772034
AGTAACA179800.058.267613
TAACAAG185250.055.9656565
GCACGCA55650.054.980272
AGCACGC55700.054.6715771
AAGAGCG37850.045.778899
AAACACG76250.044.0235375
ACAAGAG242500.042.662687
CACGCAC73800.042.440113
CAAGAGC246400.040.4571468
AACACGA88100.038.8448641
AACAAGA284150.036.588566
ACGAGCG7600.036.24969
GTAGAAA172400.032.8038521
ACACGAG107950.031.6342742
ATTAGTA73900.030.5128971
Produced by FastQC (version 0.11.2)