FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_310_rr.351000000a923e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_310_rr.351000000a923e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6595791
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA91450.069.687441
CGCACTT33650.069.590565
TAGTAAC99400.063.019272
ACGCACT41600.060.823524
GTAACAA127250.049.1121524
CGTGCGA604.947027E-448.3334963
AGCACGC51700.047.8203961
TAACAAG135200.046.2235645
AGTAACA138550.045.2635963
AAGAGCG45550.044.088069
GCACGCA55700.043.995432
AACACGA42600.041.0147746
AAACACG50000.040.3095255
ACGAGCG6350.039.9598549
ATTAGTA70650.034.9938351
ACAAGAG188200.033.4371837
ACACGAG53600.033.4088677
CACGCAC75600.033.085433
GCGGCGT1601.879509E-631.7181369
CAAGAGC195100.030.7682388
Produced by FastQC (version 0.11.2)