FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_297_rr.351000000a9221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_297_rr.351000000a9221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6915188
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT205690.2974467216220297No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC178090.2575345746203863No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG177270.25634877894859837No Hit
CTGTTGCTGGGCCCATCCCATTGTTAATCATATTGTTCTTTATTATTGTC148460.21468686028492645No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA145120.20985691206081455No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT138300.19999456269301719No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG117790.17033520997549165No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT115740.16737072079602175No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA108430.15679978620971693No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT96710.1398515846568452No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA96280.13922976497529785No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT93880.13575914349689408No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC92040.13309833369678453No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG88040.12731396456611158No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT84140.12167420466370545No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT83190.12030041699517063No Hit
GCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGTA77220.11166724606764125No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT77100.11149371499372107No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT74580.10784956244139711No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA74070.10711205537723631No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT71420.10327991082816547No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT69930.1011252333269898No Hit
GTGTCACCAAATTTTTCATCACCCATTGATAGGTGTTCTGCACCAGTTCT69670.10074924933349606No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG69430.10040218718565569No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG69380.10032988257152228No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTG100.007092016144.998268
AGCACGC4850.0109.127941
GTCGTAA203.8664398E-4108.754211
CGGTGCA12050.0104.692092
GAAACAC47250.0102.1899954
GCACGCA5450.095.782842
AAACACG50550.095.518155
CGCACTT5700.092.849775
ACGCACT5900.090.931784
GGTGCAT15050.083.3384863
GTAACAA135350.075.847454
CACGCAC6900.075.651823
AACACGA61150.074.218256
GCGGAGC25700.065.732121
ACACGAG69850.064.974177
CAGCCTT13900.064.675495
ACAAGAG159400.064.630667
CAGCGTC451.19417E-464.446472
GTAGTAA163700.064.04341
GAGCAGA26850.062.6467441
Produced by FastQC (version 0.11.2)