FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_291_rr.351000000a9214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_291_rr.351000000a9214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8276135
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA81350.051.5143741
TAGTAAC81850.049.1620332
CGCACTT22100.048.2233055
AAACACG42050.045.344195
ACACGAG35100.044.821317
ACGCACT25700.044.2900624
AACACGA41600.038.689366
AGCACGC31050.036.6603161
CACGAGC44300.035.5128338
CTGCGCG10150.034.999508145
GCACGCA33150.034.7751582
AGTAACA119150.034.1967353
GTAACAA125000.032.248134
TAACAAG132200.030.5460915
CGCGAGA1504.4029504E-528.999592145
AAGCGGT15900.027.8140747
CTATACG1050.00771125827.6193283
AGAAGCG27800.024.7748325
GAAGCGG27500.024.5178366
ACGAGCA25300.024.3572189
Produced by FastQC (version 0.11.2)