FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l02n01_s2_137_rr.351000000a9207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l02n01_s2_137_rr.351000000a9207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7648645
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA162090.21191988907839232No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT121760.15919159537408262No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT121620.15900855641750924No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC119830.1566682726156071No Hit
ATCCTTTTGTTATTCCAACTTCTATAAATCTCTTGGTTTTATAATCAAAC113020.1477647347994318No Hit
GTTAGCTATTATCGTTTTGTGAAACAATATGTTATCATCAATTCTTTTAC112410.14696720791721934No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT106450.13917497805166798No Hit
GTACTAAAGGAAGAGATGGTCTGTTAGTGGCAGATGGTGGACCCAACATT105990.13857356433721268No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC99650.13028451444667652No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAACCTCATTTAGGTTGAA95470.1248194941718435No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC94740.1238650767554253No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT91740.11994281340028201No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA91390.11948521600884862No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA91120.11913221230688573No Hit
GCTTCTGCTCCCCCCTACTCCCATGGAACGGGAACAGGCCACACAATAGA86670.11331418833008984No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC86320.11285659093865645No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG82300.10760075804276445No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT82010.10722160591843392No Hit
CCCCATAGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG81150.10609722375662617No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA81150.10609722375662617No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT77500.10132513667453517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT12400.085.361485
ACGCACT14000.078.1966554
AAACACG35500.074.745245
AGCACGC14400.072.5054241
GCACGCA16600.063.3322222
AACACGA38600.062.3564726
GAAACAC42500.062.2648854
CGGTGCA15300.062.0792
ACACGAG39700.060.8113257
ACGAGCC20350.056.6452459
CACGAGC42950.055.8721778
TAACAAG133500.052.0796555
CACGCAC20400.051.5330243
GTAACAA145250.047.7677844
AGAAGCG20300.047.142075
TAGTAAC153100.045.7947962
GCGGTGC32950.042.248991
GTAGTAA170400.041.9969831
AAGCGGT17650.040.6650437
AGTAACA175000.040.1037643
Produced by FastQC (version 0.11.2)