FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_zk-hu-0163_rr.352000000a9ae0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_zk-hu-0163_rr.352000000a9ae0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970585
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA59290.30087512083975065No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC49830.2528690718745956No Hit
GTATATAACACTTGTTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC38110.19339434736385389No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC32760.16624504905903575No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC31280.1587345889672356No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT26250.13320917392550943No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC24380.123719606106816No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT24190.12275542541935516No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA24140.12250169365949706No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG23420.11884795631754022No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC23280.1181375073899375No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC22820.11580317519924288No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC22050.11189570609742792No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC21690.11006883742644952No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC21190.10753151982786838No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAA21070.1069225636042089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCG1200.060.453723
GTACGGG502.0132196E-457.985447145
CAACTCC3600.056.374744145
CGAGTTT4150.055.939324
GGCTTCT8150.055.185791
TTCGAGT4000.054.4083442
CTAATTT400.006057762554.36136145
TCGAGTT4200.053.5447243
GTTCGAG4200.053.543361
TCTTCCG8600.052.300825
GAATAAA1405.456968E-1251.772724145
AGTTTGA4600.048.88996
CTTCCGT9950.045.204736
GTTTGAA5050.044.531117
TTCCGTG10150.044.3117457
ATATCCC1053.832618E-641.418182145
CTCGTCC1252.7261012E-740.589817145
CGAGCCC750.001485565938.656967145
CGCCGTG750.001485565938.656967145
AGGCGCC951.0307751E-438.148327145
Produced by FastQC (version 0.11.2)