FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_vww_10e3_p2_rr.352000000a9a26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_vww_10e3_p2_rr.352000000a9a26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976111
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT64280.32528537111528655No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT29980.15171212548282967No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC26620.1347090320331196No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC25210.12757380531761628No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA21320.10788867629399361No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC20770.10510543183049939No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT20370.10308125403886725No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGCATCGCATGTAT20230.102372791811796No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA20180.10211976958784197No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT20120.10181614291909714No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA22550.099.735264
CGGTGCA5300.098.547622
CGCACTT3550.091.9639745
TAGTAAC24900.090.313342
GCACGCA3650.089.435342
ACGAGCG502.2591848E-887.0592354
AGCACGC4400.075.837451
TAACAAG31650.070.8301855
ACAAGAG34350.065.893027
TTCGGAA1600.063.419594145
AAACACG12050.063.2172855
GAAACAC12000.062.876114
CGAGCGG350.003557413662.1851655
TAGAAAC17250.056.7720072
AACACGA13100.054.827386
AGTAACA41450.054.432523
GAGCATC6800.052.2782174
AGCATCT7950.052.0165255
GAACGCC701.6994296E-551.771095145
GCCGACC858.946681E-751.162025145
Produced by FastQC (version 0.11.2)