FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_vww_10e3_p1_rr.352000000a98ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_vww_10e3_p1_rr.352000000a98ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658300
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA58670.3537960562021347No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT32400.1953808116746065No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT29840.17994331544352649No Hit
GCAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATA29800.17970210456491587No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG29100.17548091418922993No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC20880.12591207863474643No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC20530.12380148344690346No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA19800.11939938491225953No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19570.11801242236024845No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT19320.11650485436893204No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT18950.11427365374178375No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT18430.11113791231984563No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT18390.110896701441235No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT18250.11005246336609781No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC17910.10800217089790749No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA17470.10534885123319063No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC16720.10082614725924138No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT16710.10076584453958873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATTG8600.0119.795575
CGGGTGG203.8716916E-4108.713394145
GAGCAGA2500.0107.370831
CGGTGCA2450.0106.6073762
GAAACAC10500.098.118264
TTCGGAA750.096.634125145
GTAACAA22200.092.487774
AGCACGC406.09527E-790.684821
GGATTGA12450.081.0021066
TAGAAAC13150.080.55232
AACACGA11750.080.270576
AACACGT1000.079.8074656
CGTGCCT702.7976057E-972.552243
ACACGAG12900.072.545677
GAGCCCC702.8212526E-972.47559145
TAACAAG28850.070.917615
CAGCCTT1950.070.691935
ACAAGAG29050.070.422997
AAACACG15200.067.779065
GAGCATC5650.066.773744
Produced by FastQC (version 0.11.2)