FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_rt-pcr_neg_ctrl_rr.352000000a990d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_rt-pcr_neg_ctrl_rr.352000000a990d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12059
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAAACACGAGCAACGGGCACGTACTCTACCGCACCTGCAGATGTTTT200.1658512314453935No Hit
CTCTATAACGCCTTGTAATTTACTACGCCATGAGCAGCGTACCTTACAGA190.1575586698731238No Hit
CTCCAAAGCTGTGGCATGTCTATATTCCAAAACGTCTGCGTTTTTGGGAA170.14097354672858448No Hit
TCCTTAAAGCGTTCCATTGAATCAGGCACAGAAGCACAGCGCGAAGCTGT160.1326809851563148No Hit
TGCCTATATTGGGGATGCGGTATATGAGGTAGCCGTACGACAGTATCTGT150.12438842358404512No Hit
GTATATGAGGTAGCCGTACGACAGTATCTGTTATCTAAAGCCAACATGCG150.12438842358404512No Hit
TATCTATGTAGCTCTCGCAAAACGAGAAGTATTTGGATATGTTGATATCG150.12438842358404512No Hit
GTGTAGCGGACATCGGAATTGCCGGTAAAGATGTCATGCTTGAGGAAGAG140.11609586201177544No Hit
CTACCGCACCTGCAGATGTTTTGGATACGGTCACTGTAACTGCAGCTGAG140.11609586201177544No Hit
TGTCTACATGCTGAATGACAATACCCAGTTTTTTCGCTTCTGTGATAATC140.11609586201177544No Hit
GCAGATACCGCGCAAAAACATCTGACTCAACCGATTATCACAGAAGCGAA140.11609586201177544No Hit
TTATAGAGAGGATCGAATAAAGATGGAAGAAGAATGGATCGCCGGTAAGC140.11609586201177544No Hit
GTCTCTGCAAGTTGACCAAGGGGGATGGTTTCCTTATCCCCCATCTCGGC140.11609586201177544No Hit
GGTAAGCACTCCGTGACGGAGGCGCTGCGTTCAGGCCGGACCATTAATAA140.11609586201177544No Hit
GGATCAGACGGTCCCGGGCGTTCAACACCAGGGAGTGGTGGCGCAAGCGG140.11609586201177544No Hit
GTATAGCAGTGGTCGGCATGATCGCATGATTGAACTGATTCGTCAGGGTA140.11609586201177544No Hit
CTCCACAACCGCTGATAACCGGCTGACCAGCTCATCAATCTGAGCATCTT130.10780330043950576No Hit
CTTCAATCTCATCCAGCAAAATCAAAAATGGATGTTCATTTTTTGCAGCC130.10780330043950576No Hit
CAATGACACCATGGGCACCTGTGCAGTCAGCCGTCCGCAAAATCGATCCA130.10780330043950576No Hit
GCTTTGGAGTGTCTGATCGGTTACCTGTATAGCAGTGGTCGGCATGATCG130.10780330043950576No Hit
ATTTTGTAGGTAATTGCGAATTTTGTTCGGCATGCTCGATACCCTGACGA130.10780330043950576No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGC259.161697E-487.01
GCGGACA259.161697E-487.06
AGCGGAC259.161697E-487.05
TAGCGGA300.001889501572.54
CGGACAT300.001889501572.57
GTAGCGG300.001889501572.53
GGACATC350.003481618662.1428578
GACATCG350.003481618662.1428579
TGTAGCG450.00941139548.3333322
CAAAGCT450.00941139548.3333324
AAAAAAA3753.8613893E-56.1866665115-119
Produced by FastQC (version 0.11.2)