FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_prvabc59b_rr.352000000a9b5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_prvabc59b_rr.352000000a9b5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548517
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA76430.2998999025707892No Hit
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC64170.25179349402024787No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC59450.233272919113351No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC51760.2030985078773263No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC44930.17629860817094803No Hit
GTCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT40230.15785651027636857No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT39100.15342255908043775No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC36870.1446723722070522No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT33940.133175489902559No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA33580.13176290368084653No Hit
GTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC32220.12642646684326608No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC31050.12183556162270058No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC30890.12120774552416169No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC30380.11920658171006902No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC27170.10661102123313285No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC27120.10641482870233944No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG26070.10229478555567807No Hit
ATAATATACAGATTATAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA25970.10190240049409129No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA25760.101078391864759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCC3750.096.64279145
CGAGTTT4200.067.363014
TCGAGTT4200.067.363013
GTTCGAG4300.065.7951351
TTCGAGT4400.064.301052
TATAAGC350.003558422762.1812322
GTCATAA10550.059.8224751
TGACGGG851.3089448E-859.691128145
CTGTATT902.0552761E-856.374958145
TTACCCG400.006057874354.361565145
TCATAAT11750.052.4791952
AGTTTGA5450.051.9127776
TGTAGGG1405.456968E-1251.772923145
ATAATAC12100.051.5607454
AATACTG12500.048.750096
TAATACT12700.048.553595
GAATTTT901.3266472E-648.321392145
GTTTGAA5950.047.548497
TCTTCCG12500.046.4286585
TGGCGGG1950.044.604362145
Produced by FastQC (version 0.11.2)