FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_pr8_f12_rr.352000000a9b82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_pr8_f12_rr.352000000a9b82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604885
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG203720.5651220496631655No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA120210.3334641743079183No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG103260.2864446438651996No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA77130.2139596686163359No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG67770.18799490136301159No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC67640.1876342795956043No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT65130.18067150547104832No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA64830.17983930139241613No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT62940.17459641569703332No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG59780.16583053273544093No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA58820.16316747968381792No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT54710.15176628380655693No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA54620.15151662258296728No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT53310.14788266477294004No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT51710.14344424302023506No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT50990.1414469532315178No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT50250.13939418317089172No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT48170.1336242348923752No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC48130.1335132743485576No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG44950.12469191111505638No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA44510.12347134513306249No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT44490.12341586486115369No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC43480.12061411112975863No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA42410.11764591658263716No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT41650.11553766625010228No Hit
GTATTCATCACCCGCCTAACAGTAAGGAACAACAGAATCTCTATCAGAAT39910.1107108825940356No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC39530.10965675742776815No Hit
ATATTATTGTGTCTCCGGGTTTTAGCAAGGTCCAGTAATAGTTCATCCTC38650.1072156254637804No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA37860.10502415472338231No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA37660.10446935200429418No Hit
CATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT37520.10408099010093248No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT36700.10180629895267115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA15550.0129.685681
GCAAAAG99650.099.369033
GCAGGGT72900.083.587539
AGCAGGC6950.082.457858
AGCAAAA121050.081.801942
GCAGGGG23550.081.012979
AGCAGGG107800.074.628128
ACGGCCC1400.072.47858145
AAGCAGG169550.070.466967
AGCGAAA29100.069.3022842
GAGCAAA144200.068.817431
CAAAAGC145650.068.185934
GGCAGCC2150.067.42193145
TACTAAT3450.067.285367
ATTAGTA3250.066.9600141
CTACTAA3400.066.1431056
TCTACTA3600.062.4684835
TGACGCC2250.057.982864145
GTTGACC2050.056.568645145
AATACTA4250.056.3275342
Produced by FastQC (version 0.11.2)