FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_pr8_d4_rr.352000000a9750.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_pr8_d4_rr.352000000a9750.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725928
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG102020.5911022939543249No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA62370.36137081036984164No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG51060.29584084619984147No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA34690.20099332069472192No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC33430.19369290028320996No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT30850.1787444203929712No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT30630.1774697438131834No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA29260.16953198511177756No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG29000.16802554915384652No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA27360.15852341464997383No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG26890.15580024195679076No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT26850.15556848257864755No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT26390.15290324973000033No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT26300.15238179112917805No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG25210.14606634807477487No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT23920.13859210812965544No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA23060.1336092814995759No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT22940.13291400336514617No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT22450.13007495098289154No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC21790.1262509212435281No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC21040.12190543290334244No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC20710.11999341803366073No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA20470.11860286176480131No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT19900.11530029062626018No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT18880.10939042648360767No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18400.10660931394588882No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA18030.1044655396980639No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA18020.10440759985352807No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA17490.1013367880931302No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG100.0070801145.07673
GAGCGAA7300.0130.164021
GCAAAAG47450.098.603243
AGCAGGC3150.087.5065848
GCAGGGG11300.086.660879
GGCAGCC1350.085.90406145
GCAGGGT34600.083.440079
AGCAAAA58000.081.1655652
AGCAGGG49850.077.413058
TATTAGT903.6379788E-1272.534141
AAGCAGG79650.070.3071067
AGCGAAA13600.069.336122
GAGCAAA70850.067.056971
CAAAAGC70950.066.2525944
TGACGCC1950.063.189045145
GCTCGGA350.003559066462.1775364
GTCGTTT1400.062.175738
GGTCGTT1450.060.0317427
CTACTAA1550.056.1603556
ATTAGTA1450.055.02591
Produced by FastQC (version 0.11.2)