FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_pr8_a2_rr.352000000a951e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_pr8_a2_rr.352000000a951e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933499
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG117540.6079134253495864No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA75080.3883115533031049No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG58740.30380155355653143No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC38540.19932774726027788No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA36980.1912594731106662No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT35630.18427731278888687No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT35600.18412215367062512No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG34080.17626075834536248No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA33670.17414025039578504No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT32400.16757184772270378No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT31590.16338255152963618No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT29070.1503491855956481No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA28540.14760804117302362No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA28530.14755632146693637No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT28410.14693568499388932No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT27150.14041900202689528No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT27140.14036728232080803No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG26230.1356607890668679No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG25930.13410919788425027No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA24260.1254720069676788No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC24190.12510996902506802No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC23380.12092067283200043No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT23130.11962768017981908No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22690.11735201311197987No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC22440.11605902045979852No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT21690.11218004250325446No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT21280.11005953455367704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7200.0138.023511
GCAAAAG55800.0103.352363
AGCAAAA65700.087.778722
GCAGGGG13900.087.671729
AGGGGGG850.085.274635145
GCAGGGT39750.082.118389
ACGGCCC451.2349992E-680.537155145
AGCAGGC2900.077.5425348
AGCAGGG58450.076.945548
TATTAGT702.8030627E-972.53791
GTTGACC903.6379788E-1272.483444145
CAAAAGC81950.070.815544
AAGCAGG95900.070.573127
ATTAGTA1550.070.1979751
AGCGAAA14600.070.053731
GAGCAAA85300.067.775751
ATCCGCC954.5838533E-1061.03869145
AAGCGGG502.0052702E-458.0318227
GGCAGCC1251.8189894E-1257.98675145
ACGTTCC502.0129865E-457.98675145
Produced by FastQC (version 0.11.2)