FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_pr8-vww_5-1_rr.352000000a9a5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_pr8-vww_5-1_rr.352000000a9a5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156198
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG53880.46601014705093763No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA32130.277893578781489No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG26650.23049685261520952No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG23430.20264695147370954No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA16330.14123878436046422No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT15790.13656830404480894No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC15790.13656830404480894No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA15710.13587638103508223No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT15430.13345465050103875No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG15040.13008152582862104No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA14830.12826522792808845No Hit
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA14690.1270543626610667No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT14340.12402719949351235No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA14340.12402719949351235No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC14120.12212441121676391No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13580.11745393090110863No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG13380.11572412337679187No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT13070.11304292171410088No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT13020.11261046983302167No Hit
GCAGTTAAACTGTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGC12620.10915085478438816No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC12350.10681561462656051No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA12320.10655614349791298No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT12280.10621018199304963No Hit
CTGAAAGAAGAATCCGATGAGGCACTTAAAATGACCATGGCCTCTGTACC11710.10128023054874684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4600.0140.337631
GCAAAAG29150.089.330113
AGCACGC259.3696214E-487.040871
TGCGGCC259.3954534E-486.98057145
GCAGGGT20200.085.464629
TCTACTA755.4569682E-1177.373025
AGCAGGG28800.076.567058
AACACGT406.646709E-572.53726
AAGCAGG47900.068.7513357
CAAAAGC39350.066.5461964
AGCGAAA9900.065.9400562
AGCAAAA41850.063.2614862
GAGCAAA43750.061.17731
CTATTCG1450.060.030799
GAGCCCC502.0125962E-457.987045145
ACCTCCC1154.0017767E-1156.726456145
CACGCAC400.006039889554.4005433
CGCAACC400.00605647254.362854145
AGGGTGT6300.054.115056
GCAGGGG8350.053.8599559
Produced by FastQC (version 0.11.2)