FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_pr8-vww_10-1_rr.352000000a9a40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_pr8-vww_10-1_rr.352000000a9a40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445798
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG75830.5244854398747266No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA46080.3187167225297033No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG39010.2698163920547684No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG28670.19829879416073337No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA24800.17153156941702782No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT23330.16136417397174432No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC22850.1580442081120599No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA21880.151335110437281No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG21850.15112761257105073No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT21810.15085094874941035No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT21090.14587099995988373No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA21070.14573266804906354No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA19550.1352194428267296No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT18960.13113865145753417No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT18500.12795701750866995No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG18280.12643536648964795No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA18020.12463705164898553No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC17900.12380706018406444No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT17510.12110958792307086No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT17140.11855044757289747No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16370.11322466900632037No Hit
GTATTCATCACCCGCCTAACAGTAAGGAACAACAGAATCTCTATCAGAAT15760.10900554572630479No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC15740.1088672138154846No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC15730.1087980478600745No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT15290.10575474582203047No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC14600.10098229489873413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6850.0137.65961
GCAAAAG37250.093.861953
GCAGGGT27650.083.950579
AACACGT353.428745E-582.901196
GGCTGGG554.405956E-879.064476145
AGCGAAA11850.078.966012
AGCAGGG39250.075.033498
AGCAAAA48400.072.988162
TGCGGCC406.675232E-572.47578145
TGACGCC702.8212526E-972.47577145
CAAAAGC50450.069.590994
AAGCAGG65200.068.310847
GCAGGGG9350.063.6166889
GAGCAAA57950.061.7088171
TCTACTA1600.058.9375655
TTATATA400.00603871854.4039082
AGCAGGC4350.051.6941348
TAGTAGA2500.049.3262063
GGTCGTT1203.605237E-948.3590247
AAGGTCG1203.605237E-948.3590245
Produced by FastQC (version 0.11.2)