FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_pr8-vww_1-5_rr.352000000a9a76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_pr8-vww_1-5_rr.352000000a9a76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635992
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA56000.34229996234700416No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG35520.2171159761172426No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC31530.1927271038000186No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT27550.1683993564760708No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT26640.162836982087932No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC19780.12090523670042397No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC19410.11864361194920268No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT17370.10617411332084754No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA16460.10061173893270872No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG203.857289E-4108.815221
GAGCGAA1750.0103.6335451
AGCACGC750.096.724651
CGGTGCA4450.096.184192
GCACGCA800.090.682132
GTAACAA19100.089.2578054
AGGATTG10550.088.705175
AGCGGGG259.3974266E-486.97761145
CACGCAC850.085.3478853
GAGCAGA2950.083.6094361
GCGGAGC2500.072.543481
GAAACAC11650.067.252674
ACGTGCA1200.066.500232
AGCATCT10400.065.570165
GGATTGA14700.062.675546
TAACAAG27700.062.3316885
GAGCCCC1051.6370905E-1162.126858145
TAGAAAC14850.061.065412
CGCACTT1200.060.4547545
GAGCATC10450.060.39694
Produced by FastQC (version 0.11.2)