FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_pr8-vww_1-1_rr.352000000a9a69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_pr8-vww_1-1_rr.352000000a9a69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047643
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA26610.25399873811976026No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG26230.25037154832323605No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG19070.18202765636767487No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA15210.14518304422403433No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC13110.12513804798008482No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG12770.12189266763582632No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT12090.11540190694730934No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT11960.1141610262274458No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11600.11072474115705445No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2250.0132.228321
ATCCGCC301.6023227E-596.63228145
ACGTGCA800.090.705412
GAGCAGA1150.088.339171
GTAACAA10900.085.8788764
GCAGGGT11100.077.794199
AGCAGGG15550.076.064218
ACGCACT300.00192896972.564324
GCACGCA300.00192896972.564322
GCGGAGC903.6379788E-1272.564321
CGGTGCA3900.070.703692
GCAAAAG18800.070.6344153
TCTACTA851.6916601E-1068.295835
CGGAGCA754.822141E-967.72672
CACGCAC350.003554041562.197993
CAAAAGC22000.061.0199934
TAACAAG16900.056.6774565
AAACACG5850.055.818715
AGCACGC400.006029772654.423241
TTGCGCC400.00605948854.35566145
Produced by FastQC (version 0.11.2)