FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_pr8-vww_1-10_rr.352000000a9a83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_pr8-vww_1-10_rr.352000000a9a83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274834
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA51720.40569987935684176No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG44190.3466333655989721No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT25180.1975159118755854No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC23980.1881029216352874No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT22340.17523850164021354No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC15680.1229964058065599No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC15590.12229043153853757No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14990.11758393641838859No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT14360.11264211654223218No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA13710.10754341349540411No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA13620.10683743922738176No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT13170.10330756788727002No Hit
GTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGGG13100.10275847678991933No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT12850.10079743715652391No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCG100.0070968024144.96094145
CGGTGCA4350.0106.738512
AGGATTG7100.0100.141765
GAGCGAA800.099.754651
AGCACGC750.096.731771
GTAACAA16800.095.871874
GCGGAGC1250.087.05861
GCACGCA850.085.351572
AGCATCT7650.080.612985
TTCGGAA2000.079.72851145
GGATTGA8850.073.781376
AACACGT702.7976057E-972.551696
ACGCACT1000.072.551684
GAGCATC7600.072.551684
CACGCAC1000.072.5488363
GAGCAGA2300.072.5488361
CGGAGCA1300.072.5488362
CGCACTT1050.069.096845
TAACAAG23750.069.038665
GAAACAC8600.064.959074
Produced by FastQC (version 0.11.2)