FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_pr8-vww_1-100_rr.352000000a9a90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_pr8-vww_1-100_rr.352000000a9a90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675012
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA60540.36143024646987604No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG38280.22853567616232004No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC35090.20949103648212672No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT29620.17683455402110554No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT27470.1639988250830442No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC23030.1374915522993268No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC21420.12787968086198787No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT20920.12489462762057824No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA20250.12089465627708935No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20230.12077525414743297No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT18550.11074547525629667No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA18480.11032756780249933No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT18220.10877534011696634No Hit
GTATTACCCAAGGGTTGTTACTAATACATTCTTCTATTCCAGAATACATT16900.10089479955964495No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAG100.007095333144.97232145
AGGATTG7700.0117.760335
CGGTGCA5200.0112.988812
ACGTGCA1500.096.71472
GATCTCC607.2759576E-1296.64821145
GTAACAA22000.093.972754
TTCGGAA850.093.80562145
GAAACAC8850.087.704174
GCTCTGG259.39498E-486.9834145
GAGCAGA2550.082.489491
GAGCATC6550.080.846514
GGATTGA11050.079.433346
TAGAAAC11050.076.1464162
AGCATCT8450.073.828075
AGCACGC702.8030627E-972.533851
CGCACCC502.309538E-672.48616145
AGTAGCC801.0004442E-1072.48616145
CACGTGC2600.069.7440951
ACAAGAG29900.068.175437
ACGGGAA651.4004763E-766.9103145
Produced by FastQC (version 0.11.2)