FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_pa-259459_rr.352000000a9ab9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_pa-259459_rr.352000000a9ab9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571681
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA64040.24901999898121113No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC50610.19679734772703145No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT38090.1481132379949146No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC34950.1359033254902144No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT34460.13399795697833441No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA32950.1281263111560104No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC28160.10950036182559189No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC27220.10584516508851605No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC26390.1026177041398214No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCACGCGATGGCAG25960.10094564605796752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTT3450.067.287553
TTCGAGT3500.066.325012
CGAGTTT3600.064.483914
GTTCGAG4700.054.0182081
CAACTCC5850.049.557632145
TCTTCCG9000.045.1387335
CTCGTCC2000.043.486824145
TTCCGTG9950.040.82987
TACGCGG907.499103E-540.26558145
CAGTTCG2005.456968E-1239.8994145
CTTCCGT10300.039.4416126
ACAGTTC2057.2759576E-1238.9262584
GGCTTCT10350.038.5471531
CCGAGGG951.0311791E-438.146336145
TATAGGA18200.037.865842
ATATAGG18650.036.9500271
CCGACGC800.00204024136.239017145
TCCGACC850.0027468634.10731145
GCCGACC1501.1375014E-633.823086145
GTTTGAA6900.033.6444327
Produced by FastQC (version 0.11.2)