FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_p6-740_rr.352000000a9aad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_p6-740_rr.352000000a9aad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058125
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTAGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAA35460.17229274218038262No Hit
ATGTTGGATGAGGGGGTAGAACCAGATGACGTCGATTGCTGGTGCAACAC34420.1672395991497115No Hit
GTATAGAGTGATGACTCGCAGACTGCTAGGTTCAACACAAGTTGGAGTGG26560.1290494989371394No Hit
CTTAATACATTCACCAACCTGGTGGTGCAGCTCATTCGGAACATGGAGGC24830.120643789857273No Hit
CTCTATACACTCCATCTGTGGTCTCCCCCTTTTTTACTTCCTTGGGAGCA22860.11107197084725175No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATGGGCTTGGTTTCC21180.10290920133616763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAGT2250.077.382612
TCGAGTT2450.068.104593
GTTCGAG2500.066.739251
GTATCCC1009.094947E-1265.234406145
TAACGTG502.0049074E-458.0341347
CTATAAA701.6990194E-551.773335145
AACGTGC604.9319793E-448.3617748
TCTTCCG7550.040.356825
TAGACCC1451.9335857E-839.990437145
TGGCGGG1651.369699E-939.536003145
CTTCCGT7800.039.063336
TCCGACC750.001485495138.657425145
GCGAGGG750.001485495138.657425145
GGTTCCC1553.480818E-837.41041145
CGGTGGG2800.036.241333145
CAGACGG1406.626524E-736.241333145
CTATATG22000.035.6118551
TCTCGGG2252.0008883E-1135.435974145
TGATATT1458.7206536E-734.991634145
GACGAAA1458.7206536E-734.991634145
Produced by FastQC (version 0.11.2)