FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_ny238_plasmid_rr.352000000a9b9f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_ny238_plasmid_rr.352000000a9b9f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202035
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG58570.2659812400801985No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG50660.2300599218450206No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA44040.1999968211222801No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG40390.18342124443980226No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC40130.18224051842954359No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT31840.14459352371783374No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT31050.14100593314820153No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT30410.13809953066141092No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA29080.13205966299354915No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT27660.12561108247598243No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT26290.11938956465269626No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT25370.11521161107793472No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT24520.11135154527516593No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC24420.11089741988660488No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT23740.10780936724438986No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT23700.10762771708896543No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG23660.10744606693354102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACA1402.7102942E-1046.6369744
AATATTT2300.044.116524145
TCCTCCC4850.043.33685145
TACTGAT17800.043.2008067
ATACATC12200.041.6249853
GTCGGCC1601.0149961E-940.76839145
TATACTG32550.034.991665
TACCGCC2302.7284841E-1134.662983145
ACCGGCC1905.4496923E-934.33128145
CCGTTCC2154.8930815E-1033.710297145
CATATTA8350.032.146315
CCATATT8750.031.5058674
TACATCA16750.031.1841244
TATATAT30550.031.1068632
TATCCCG28250.030.0457675
CATATAC29050.029.9675333
TATTACT6100.029.730782
GTATATA44750.029.6649931
GCACACC2951.4551915E-1129.48223145
ATCCCGT29200.029.3166966
Produced by FastQC (version 0.11.2)