FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_mr766a_rr.352000000a9b4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_mr766a_rr.352000000a9b4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484716
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA50990.20521459997842809No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG47030.18927716487518093No Hit
GAATGGTTTTGGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCA45540.18328050368734292No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA43700.17587523081108666No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATGAGCTTGGTTTCC32480.13071916468521955No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG31090.1251249639797868No Hit
TACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAA27910.11232672063930044No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA27320.10995220379310955No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT25040.10077610479427025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGGG3050.083.17605145
TCGAGTC3450.063.0786363
TTCGAGT3450.063.0773662
CGAGTCT3550.061.303014
GTTCGAG3550.061.2980651
GCGACAG1102.5465852E-1159.347671
CGACAGT1800.056.4169271
GCTAATA400.006040049354.4020351
CCTTATG4200.053.538511
GAATAAA3000.053.153454145
AGTTGCC1950.052.03835145
GAAGCGA7000.049.743016
AAGCGAG7000.049.743017
AGTCTGA7000.049.7391
TGAAGCG6800.049.0724565
GCGTCAG604.9343985E-448.3573651
TTATGGG4700.047.8458183
TCTTCCG10250.046.002185
GGCTTCT10550.045.3781
CAGTTCG2250.045.1371774
Produced by FastQC (version 0.11.2)