FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_mex-i-44_rr.352000000a9b0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_mex-i-44_rr.352000000a9b0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746083
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA71390.2599702922307883No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC60500.2203138069752444No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC46140.16802114138574833No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT39260.14296727374955528No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC38900.14165631555928937No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC38850.14147423803286355No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC38470.14009044883202731No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC37350.13601191224008888No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA36410.13258885474328344No Hit
ATATCAGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTA35910.13076807947902522No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT35030.12756351501393073No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC34120.1242497040329808No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC30930.11263315784701337No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG30850.11234183380473206No Hit
ATAATATACAGATTGTAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA28760.1047309932001327No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC28540.10392985208385909No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTT203.8712172E-4108.71867145
CTAATAT1400.082.901273
CGAGTTT3300.076.934894
TAATATT1700.072.538614
GTTCGAG3650.071.542321
GGCTTCT8300.069.914181
TTCGAGT3650.069.55632
TCGAGTT3800.066.8118743
GTCTAAT1850.066.654671
TCTTCCG10150.057.173295
TTCCGTG12600.055.8432127
GTTTGAA4650.054.5989537
TGTAGGG1206.002665E-1154.359337145
CGGCTCC1750.053.841625145
CTTCCGT10800.053.73236
AGTTTGA4750.053.4495056
CTCGTCC2000.050.735382145
TTTCCGT2750.050.1175846
CGAATAA1457.2759576E-1250.0266275
TTTTCCG2800.049.2226265
Produced by FastQC (version 0.11.2)