FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_mex-2-81_rr.352000000a9afd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_mex-2-81_rr.352000000a9afd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365759
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA70930.29981921235425923No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC54570.23066593004612895No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC47170.19938632802411405No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC43300.18302794156124946No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC43050.1819711982496949No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT33940.14346347197664683No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT31930.13496725575174817No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG29870.12625969086453862No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC28870.1220327176183204No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC28790.12169455975862292No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC28540.12063781644706835No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA27060.11438189604266538No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC26870.11357877112588392No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG26620.11252202781432935No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAA26440.11176117263001006No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA26310.11121166610800172No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC26070.11019719252890933No Hit
GTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGA25610.10825278483564894No Hit
GCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGT24720.10449077864651472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCC4800.081.538956145
TATCACC1050.069.027695145
GGCTTCT12500.067.317551
TCTTCCG13300.064.359215
CGAGTTT4250.063.1528744
TTCGAGT4300.062.418542
TCGAGTT4400.060.9999353
GTCTAAA606.8201207E-660.399227145
GTTCGAG4550.058.988951
ATTGATA2250.058.0323681
CTTCCGT14800.057.836326
TGTAGGG1154.0017767E-1156.722755145
ACAGTTC2100.055.2689254
TTCCGTG15700.054.518557
TGACGGG1353.6379788E-1253.6882145
CAGTTCG2300.050.4629335
CTCGTCC1301.3278623E-1050.177822145
TTGATAG2650.049.2727662
CTACAAG450.00961952148.3603131
TTTCGCC450.00965174848.319386145
Produced by FastQC (version 0.11.2)